While covalent modifications of proteins and DNA and their effect on gene expression have been studied for many years, the regulatory consequences of RNA modifications have been less studied. Recent work has established that methylation of adenosine at the N6-position (N6-methyladenosine, m6A) plays fundamental roles in genetic control throughout eukaryotes. In our work, we have focused on identification of  N7-methylguanosine (m7G) modifications, which are best known from modification of tRNAs